Learning objectives
<br />Advanced competences on mass spectrometry thecniques and their application in the chemical and biological fields.
Course unit content
<br />Low and high resolution Mass analyzer: linear quadrupole (Q), triple quadrupole (QqQ), ion trap (IT), time of flight (TOF), quadrupole-time of flight (QTOF), double focusing sector, Ion cyclotron resonance-fourier transform analyser (FTICR). MS and MSn analysis. Aquisition signal modes. Qualitative and quantitative analysis. <br />Electron impact. Chemical ionization. Atmospheric pressure chemical ionization (APCI). Atmospheric pressure photoionization (APPI). Electrospray: principles and applications. Matrix effect in electrospray.<br />Matrix Assisted Laser Desorption Ionization (MALDI)-TOF-MS. Principles and applications.<br />Surface Enhanced Laser Desorption Ionization (SELDI)-TOF-MS. Principles and applications.<br />Inductively coupled plasma (ICP)-MS. Sample preparation methods for the mass spec analysis. Hyphenated techniques: liquid chromatography-mass spectrometry. Capillary electrophoresis-mass spectrometry. Liquid chromatography-ICP-MS.<br />Analytical data quality: evaluation of analytical data reliability in mass spectrometry.
Bibliography
<br />J.H. Gross, Mass Spectrometry. A textbook. Ed. Springer.